cx37 antibody cat. no. #42-4400  (Thermo Fisher)

Journal: International Journal of Molecular Sciences

Article Title: New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model

doi: 10.3390/ijms25116074

Figure Lengend Snippet: Expression of Cx37 in oocytes and CCs after cryopreservation and in vitro maturation in different co-culture systems. ( A ) Relative mRNA expression of Cx37 in oocytes; ( B ) relative mRNA expression of Cx37 in CCs; ( C – S ) immunofluorescent staining of Cx37 (green) and nucleus counterstaining with DAPI (blue). ( C , D ) ImmCOCs; ( E ) negative control; ( F , G ) COCs; ( H , I ) cryoCOCs; ( R ) fCCs; ( S ) cryoCCs. Descriptions of the groups can be found in (Experimental Design). Different subscript letters on the graph mean statistically significant difference between the groups ( p ≤ 0.05).

Article Snippet: As primary antibodies, we used Cx37 antibody Cat. No. #42-4400 (ThermoFisher Scientific, Waltham, MA, USA) at a 1:100 ratio.

Techniques: Expressing, In Vitro, Co-Culture Assay, Staining, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model

doi: 10.3390/ijms25116074

Figure Lengend Snippet: Primers used for qRT-PCR of genes in bovine oocytes and cumulus cells.

Article Snippet: As primary antibodies, we used Cx37 antibody Cat. No. #42-4400 (ThermoFisher Scientific, Waltham, MA, USA) at a 1:100 ratio.

Techniques: TaqMan Assay

Journal: Journal of Developmental Biology

Article Title: Effect of Cyclic Adenosine Monophosphate on Connexin 37 Expression in Sheep Cumulus-Oocyte Complexes

doi: 10.3390/jdb12020010

Figure Lengend Snippet: Antibody information.

Article Snippet: Antibody incubation and stain processing: for sections, non-specific antibody binding was blocked in 5% normal donkey serum (NDS, O17-000-121, Jackson Laboratories, Bar Harbor, ME, USA) at room temperature for 1 h. After incubation with Cx37 antibodies (DF4079, Affinity BioSciences, Cincinnati, OH, USA) at 37 °C for 1 h, we performed a subsequent incubation for 1 h at 37 °C with the corresponding Alexa Fluor-linked secondary antibodies (Donkey-Antibody-Rabbit, Alx647, Jackon Immuno Research, West Grove, PA, USA).

Techniques: Molecular Weight

Journal: Journal of Developmental Biology

Article Title: Effect of Cyclic Adenosine Monophosphate on Connexin 37 Expression in Sheep Cumulus-Oocyte Complexes

doi: 10.3390/jdb12020010

Figure Lengend Snippet: Expression of the Cx37 protein and GJA4 gene in sheep COC. Ovine COC section samples were stained for Cx37 using an Alx647 conjugated secondary antibody (Red) and nucleus using a DIPI (blue). ( A ) represents the combination of Cx37 and DIPI; ( a 1 ) and ( a 2 ) illustrate Cx37 and DIPI, respectively; ( a ) illustrates COCs morphology with normal light; ( B ), ( b 1 ), and ( b 2 ) are the negative comparison of A, ( a 1 ), and ( a 2 ) respectively; ( b ) shows COCs morphology with normal light; ( C ) illustrates the PCR electrophoresis results for the GJA4 gene. The size of the amplified product fragment of GJA4 gene in sheep COCs, CCs, and oocyte is 155 bp.

Article Snippet: Antibody incubation and stain processing: for sections, non-specific antibody binding was blocked in 5% normal donkey serum (NDS, O17-000-121, Jackson Laboratories, Bar Harbor, ME, USA) at room temperature for 1 h. After incubation with Cx37 antibodies (DF4079, Affinity BioSciences, Cincinnati, OH, USA) at 37 °C for 1 h, we performed a subsequent incubation for 1 h at 37 °C with the corresponding Alexa Fluor-linked secondary antibodies (Donkey-Antibody-Rabbit, Alx647, Jackon Immuno Research, West Grove, PA, USA).

Techniques: Expressing, Staining, Comparison, Electrophoresis, Amplification

Journal: Journal of Developmental Biology

Article Title: Effect of Cyclic Adenosine Monophosphate on Connexin 37 Expression in Sheep Cumulus-Oocyte Complexes

doi: 10.3390/jdb12020010

Figure Lengend Snippet: The effect of cAMP on the expression of Cx37 in sheep COC. ( A ) illustrated the effect of the cyclic adenosine monophosphate modulator on the level of cAMP in sheep COCs; ( B ) illustrates the effect of the cyclic adenosine monophosphate on the expression of the Cx37 gene in sheep COC; ( C ) illustrates the effect of the cyclic adenosine monophosphate on the expression of the Cx37 protein in sheep COC. F + I stands for Forskolin + IBMX, indicating the processing group. The experiment was repeated three times; * indicates a significant difference ( p < 0.05).

Article Snippet: Antibody incubation and stain processing: for sections, non-specific antibody binding was blocked in 5% normal donkey serum (NDS, O17-000-121, Jackson Laboratories, Bar Harbor, ME, USA) at room temperature for 1 h. After incubation with Cx37 antibodies (DF4079, Affinity BioSciences, Cincinnati, OH, USA) at 37 °C for 1 h, we performed a subsequent incubation for 1 h at 37 °C with the corresponding Alexa Fluor-linked secondary antibodies (Donkey-Antibody-Rabbit, Alx647, Jackon Immuno Research, West Grove, PA, USA).

Techniques: Expressing

Journal: Journal of Developmental Biology

Article Title: Effect of Cyclic Adenosine Monophosphate on Connexin 37 Expression in Sheep Cumulus-Oocyte Complexes

doi: 10.3390/jdb12020010

Figure Lengend Snippet: Effect of cAMP-PKA on Cx37 protein. ( A ) illustrates the effect of cyclic adenosine monophosphate modulator on the level of cAMP in sheep COCs; ( B ) illustrates the cyclic adenosine monophosphate regulating the expression of the Cx37 protein in sheep COCs. F + I and RP + F + I stand for Forskolin + IBMX and RP-cAMP + Forskolin + IBMX, respectively, representing the processing group. Forty COCs per group. Different letters indicate significant differences ( p < 0.05).

Article Snippet: Antibody incubation and stain processing: for sections, non-specific antibody binding was blocked in 5% normal donkey serum (NDS, O17-000-121, Jackson Laboratories, Bar Harbor, ME, USA) at room temperature for 1 h. After incubation with Cx37 antibodies (DF4079, Affinity BioSciences, Cincinnati, OH, USA) at 37 °C for 1 h, we performed a subsequent incubation for 1 h at 37 °C with the corresponding Alexa Fluor-linked secondary antibodies (Donkey-Antibody-Rabbit, Alx647, Jackon Immuno Research, West Grove, PA, USA).

Techniques: Expressing

Journal: Microcirculation (New York, N.Y. : 1994)

Article Title: K IR channel regulation of electrical conduction along cerebrovascular endothelium: Enhanced modulation during Alzheimer’s disease

doi: 10.1111/micc.12797

Figure Lengend Snippet: Presence of connexins and inward-rectifying K+ (KIR) channels in cerebrovascular endothelium of young and aged 3xTg-AD mice. Endothelial tube immunofluorescence: (A) Immunofluorescent image of endothelial Cx37 in a young, Pre-AD mouse (1 to 2 mo). (B) As in A for Cx40. (C) As in B for KIR2.1. (D, E, F) As in A, B, and C respectively for aged, AD mice (14 to 16 mo). Images represent n=3 isolated cerebral endothelial tubes of 3 different animals (1 male & 2 females or 2 males & 1 female) per group. Scale bar = 50 μm.

Article Snippet: The endothelial tube was incubated overnight at 4°C with one of the following primary antibodies: rabbit polyclonal primary antibody for Cx37 (42-4400, ThermoFisher), Cx40 (36-4900, Invitrogen, ThermoFisher), or K IR 2.1 (APC-026; Alomone Labs, Jerusalem, Israel).

Techniques: Immunofluorescence, Isolation

Journal: Cell Communication and Signaling : CCS

Article Title: NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions

doi: 10.1186/1478-811X-12-33

Figure Lengend Snippet: NO inhibited myoendothelial signal propagation in co-cultured HUVEC and HUVSMC. A/B depicts the immunohistochemical staining of Cx37 (EC, red), CD31 (EC, blue) and α-smooth-muscle actin (SMC, red) in a co-culture of endothelial and smooth muscle cells ( A , scale bar 30 μm) and an area with endothelial cells only within the same co-culture ( B , scale bar 30 μm). C depicts the number of responding cells in co-cultures of HUVEC and HUVSMC. Treatment with NO (15 min, 2 μM SNAP) significantly reduced the Ca 2+ i signal transfer from SMC to EC and also from EC to SMC whereas it did not affect the signal transfer from EC to EC and from SMC to SMC (n = 13-21, w = C = 5; p < 0.05). In the remaining responding cells, the time delay (D) was significantly increased from SMC to EC whereas the signal spread faster from SMC to SMC after exposure to NO. The amplitude of the Ca 2+ i increase (in the remaining responding cells) was unchanged in all cells (n = 17-106, w = 14-21, C = 6; *: p < 0.05, con vs. NO, NG). E . The decrease of the mechanically induced calcium rise in the initial stimulated cells was reduced by incubation with SNAP (15 min, 2 μM).

Article Snippet: After washing twice, and perfusing the for 5 min with PBS the vessels were incubated and perfused for 2 hours with PBS containing 1% BSA and 0.3% Triton-X100, washed and perfused again with PBS-1% BSA and incubated overnight with the antibodies against Cx37 (10 μg/ml Alpha Diagnostics, Cat. No. Cx37A11-A), Cx43 (see above), Cx40 (3.3 μg/ml polyclonal rabbit anti mouse-Cx40, Alpha-Diagnostics, Cat. No. Cx40-A) or Cx45 (2.5 μg/ml polyclonal rabbit anti Cx45 c-Term, Zymed, Cat. No. 40–7000) and against CD31 (2.5 μg/ml monoclonal rat anti mouse-CD31, Abcam, Cat. No 56299; antibodies were applied from the endothelial and smooth muscle side).

Techniques: Cell Culture, Immunohistochemical staining, Staining, Co-Culture Assay, Incubation

Journal: Cell Communication and Signaling : CCS

Article Title: NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions

doi: 10.1186/1478-811X-12-33

Figure Lengend Snippet: Distribution of Cx37 within the internal elastic lamina. A . Representative 2-photon-image of a small resistance artery for visualization of the internal elastic lamina and the connexins. Cx37 (red) located in the small holes (dark dots) within the internal elastic lamina (autofluorescence, green). Arrows indicate some of the holes in which Cx37 could be detected; scale bar: 25 μm. B depicts the summary of n = 7-10 experiments (3 vessels each) revealing the percentage of holes in the internal elastic lamina that contain the different vascular Cx (*: p < 0.05 vs. Cx40; #: p < 0.05 vs. Cx45, NG).

Article Snippet: After washing twice, and perfusing the for 5 min with PBS the vessels were incubated and perfused for 2 hours with PBS containing 1% BSA and 0.3% Triton-X100, washed and perfused again with PBS-1% BSA and incubated overnight with the antibodies against Cx37 (10 μg/ml Alpha Diagnostics, Cat. No. Cx37A11-A), Cx43 (see above), Cx40 (3.3 μg/ml polyclonal rabbit anti mouse-Cx40, Alpha-Diagnostics, Cat. No. Cx40-A) or Cx45 (2.5 μg/ml polyclonal rabbit anti Cx45 c-Term, Zymed, Cat. No. 40–7000) and against CD31 (2.5 μg/ml monoclonal rat anti mouse-CD31, Abcam, Cat. No 56299; antibodies were applied from the endothelial and smooth muscle side).

Techniques:

Journal: Cell Communication and Signaling : CCS

Article Title: NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions

doi: 10.1186/1478-811X-12-33

Figure Lengend Snippet: Location of vascular Cx across the vessel wall. A . Confocal images of triple (Cx, α-actin, CD31) immunohistochemical stainings of Cx40, Cx45, Cx37, and Cx43 in small resistance arteries. Left panel: Overlay of a z-series in xy-direction, right panel: Cross section (slice) of the z-stack in yz-direction along the yellow lines in the z-stack. The arrows depict the Cx expression in ECL (yellow) or beyond EC and within SMC (SMCL, white), scale bars: 10 μm. B . Summary of the Cx distribution within the ECL and SMCL for all Cx (n = 4-8, at least 3 vessels each; *: p < 0.001).

Article Snippet: After washing twice, and perfusing the for 5 min with PBS the vessels were incubated and perfused for 2 hours with PBS containing 1% BSA and 0.3% Triton-X100, washed and perfused again with PBS-1% BSA and incubated overnight with the antibodies against Cx37 (10 μg/ml Alpha Diagnostics, Cat. No. Cx37A11-A), Cx43 (see above), Cx40 (3.3 μg/ml polyclonal rabbit anti mouse-Cx40, Alpha-Diagnostics, Cat. No. Cx40-A) or Cx45 (2.5 μg/ml polyclonal rabbit anti Cx45 c-Term, Zymed, Cat. No. 40–7000) and against CD31 (2.5 μg/ml monoclonal rat anti mouse-CD31, Abcam, Cat. No 56299; antibodies were applied from the endothelial and smooth muscle side).

Techniques: Immunohistochemical staining, Expressing